384 well plate  (Greiner Bio)

Journal: bioRxiv

Article Title: Using a GPT-5-driven autonomous lab to optimize the cost and titer of cell-free protein synthesis

doi: 10.64898/2026.02.05.703998

Figure Lengend Snippet: OpenAI’s GPT-5 proposes a batch of experimental designs in 384-well plate format and validates them using a Pydantic schema. Designs are then programmatically converted to Catalyst protocol runs for execution on Ginkgo’s RAC-based cloud laboratory. After experiments are executed, (meta)data and metrics are returned to GPT-5 for analysis, interpretation, hypothesis generation, and design of the next round of experiments.

Article Snippet: Next, the sfGFP protein standards and plasmid DNA template were liquid transferred (constant 2 μL volume stamp), using the Bravo 384 liquid handler (Agilent), from a pre-made, reusable 384-well source plate (Greiner, cat. #781281) into the pre-filled (with variable reaction components) 384-well destination plate.

Techniques:

Journal: bioRxiv

Article Title: Using a GPT-5-driven autonomous lab to optimize the cost and titer of cell-free protein synthesis

doi: 10.64898/2026.02.05.703998

Figure Lengend Snippet: (A) CFPS reaction cost ($/L) versus protein titer (g/L) across Steps 0-5 of experimentation in 384-well plate reaction geometry (Pareto frontier plotted on a log-log scale). Reactions representing the minimum specific cost ($/g) for each step are highlighted with larger dots. Star: SOTA in 384-well plates as reported by Olsen et al . (2025). 42 (B) Steps 3-5 of panel A replotted on a linear scale. (C) Best specific cost ($/g) obtained in 384-well plate reaction geometry at each step. Dotted line: SOTA in 384-well plates as reported by Olsen et al .

Article Snippet: Next, the sfGFP protein standards and plasmid DNA template were liquid transferred (constant 2 μL volume stamp), using the Bravo 384 liquid handler (Agilent), from a pre-made, reusable 384-well source plate (Greiner, cat. #781281) into the pre-filled (with variable reaction components) 384-well destination plate.

Techniques:

Journal: bioRxiv

Article Title: Using a GPT-5-driven autonomous lab to optimize the cost and titer of cell-free protein synthesis

doi: 10.64898/2026.02.05.703998

Figure Lengend Snippet:

Article Snippet: Next, the sfGFP protein standards and plasmid DNA template were liquid transferred (constant 2 μL volume stamp), using the Bravo 384 liquid handler (Agilent), from a pre-made, reusable 384-well source plate (Greiner, cat. #781281) into the pre-filled (with variable reaction components) 384-well destination plate.

Techniques: